Transfection Slideshare 68431

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File: Transfection Slideshare 68431 | Lec No 6(4)

two different methods transfection in vitro packaging transfection equivalent to transformation the only difference being that phage dna rather than a plasmid is involved the purified phage dna or recombinant ...

Filetype Power Point PPTX | Posted on 29 Aug 2022 | 4 years ago

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...Two different methods transfection in vitro packaging equivalent to transformation the only difference being that phage dna rather than a plasmid is involved purified or recombinant molecule mixed with competent e coli cells and uptake induced by heat shock standard method for introducing double stranded rf form of an m cloning vector into vectors requires number proteins coded genome but these can be prepared at high concentration from infected defective strains single strain system carries mutation cos sites so are not recognized endonuclease normally cleaves catenanes during replication this means cannot replicate though it does direct synthesis all needed accumulate bacterium cultures mutated protein preparation then used molecules second both carry gene one components coat d neither able complete infection cycle because absence product capsid structure made instead products other genes mix therefore combining lysates mixture now contains necessary systems formation particles achie...

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Posted on: 29 Aug 2022 | 4 years ago
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