Transfection Slideshare 67002 | Transfection Presentation 1

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File: Transfection Slideshare 67002 | Transfection Presentation 1

transfection to transfer dna into cells either eukaryotic or prokaryotic not through use of a viral vector the approaches for eukaryotic and prokaryotic cell transfection are slightly different we have ...

Filetype Power Point PPTX | Posted on 28 Aug 2022 | 4 years ago

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...Transfection to transfer dna into cells either eukaryotic or prokaryotic not through use of a viral vector the approaches for and cell are slightly different we have two things worry about transforming that want put stability transient gene products expressed in target however nucleic acids do integrate host genome results high expression levels persist hours when rna is transfected following stable initially interest has be introduced subsequently nucleus finally it integrated chromosomal isolate stably selection used sequence randomly transformation first most easily taken up if plasmid form as opposed linear although certain can take plasmids nicked been re ligated this lower efficiency supercoiled gives highest generally will an antibiotic resistance marker i e tetracycline kanamycin ampicilin which stop bacterial growth means so were successfully transformed identified then competent able some bacteria naturally made lab known artificial competence get already ordered from company...

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Posted on: 28 Aug 2022 | 4 years ago
Tag: download pptx, transfection, presentation,
Filetype: PPTX
File size: 2.39 MB
Views: 97x
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Uploaded by: Andy yahya

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