Journal of Islamabad Medical & Dental College (JIMDC); 2015:4(3):103-105 Original Article Importance of pH of Fixatives Used for Fixation of Histopathology Specimens – An Un-Recognized Issue Usman Hassan, Muhammed Kashif Baig, Sajid Mushtaq Department of Pathology, Shaukat Khanum Memorial Cancer Hospital & Research Centre, Lahore Abstract sensitive techniques are being introduced in histopathology Objective: Adequate fixation of the surgical specimens which have very strong impact on diagnosis. So, a good is very important to ensure optimal histopathology, fixation is not only necessary for light microscopic immunohistochemistry and genetic tests results. To examination but also for more advanced and ...
MODULE Cytology : Staining Methods Histology and Cytology 25 Notes CYTOLOGY : STAINING METHODS 25.1 INTRODUCTION Consistency and reliability are most important in cytological interpretation. Cytologists rely heavily on the quality and appearance of the stain. The Papanicolaou stain is recommended for the staining of alcohol fixed cytology slides. Romanowsky stains may also be used for wet fixed slides, but are primarily applied to air-dried smears. Special stains are used as per requirements: Modified Ziehl Neelson (for acid fast bacilli), Gram staining (Bacteria), Mucicarmine (mucins), PAS (for glycogen, fungal wall, lipofuscin, etc), Oil red O (lipids), Perl’s Prussian ...
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