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...The journal of animal plant sciences page saleemet al issn j anim sci in vitroantimicrobial susceptibility testing leaves methanol extract and latex ofeuphorbia helioscopiausing agar well diffusion broth dilution methods u saleem m b ahmad k hussain n i bukhari a anjum university college pharmacy punjab lahore pakistan faculty pharmaceutical gc faisalabad department microbiology veterinary corresponding author ahmadbprof gmail com abstract antimicrobial is necessary to claim antibacterial activity new chemicals euphorbia helioscopia has great medicinal importance because its traditional uses number pharmacological activities various factors affect chemical composition which may ultimately modify was tested by adopting clsi guidelines method macrodilution were used validate standardized e showed no coli ai mic mg ml s enterica staph aureus found bactericidal against as mbc same whereas relatively dose dependent bacteriostatic at however upto highest employed key words introduction antif...