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...Appl sci converg technol http dx doi org asct review paper an overview of techniques in enzyme immobilization a b c hoang hiep nguyen and moonil kim hazards monitoring bionano research center korea institute bioscience biotechnology kribb gwahangno yuseong gu daejeon department nanobiotechnology university science technology ust gajeongno pathobiology college veterinary medicine nursing allied health cvmnah tuskegee al usa received september revised november accepted abstract immobilized enzymes have become the subject considerable interest due to their excellent functional properties such as reusability cost effectiveness optimality during past decades is not only used industrial processes but also component products for medical diagnostics therapy food industry bio energy biomaterial detection this new methods are introduced advantages disadvantages variety will be discussed keywords i introduction development multi reaction system over biochemical biophysical most relatively unstabl...