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...Atechnical review and guide to rna uorescence in situ hybridization alexander p young daniel j jackson russell c wyeth department of biology st francis xavier university antigonish ns canada geobiology georg august universitat gottingen germany abstract fish is a powerful tool visualize target messenger transcripts cultured cells tissue sections or whole mount preparations as the technique has been developed over time an ever increasing number divergent protocols have published there now broad selection options available facilitate proper preparation post background removal achieve optimal results here we technical aspects examining most common methods associated with different sample types including cytological mounts discuss application commonly used reagents for washing provide explanations functional roles each reagent also probe necessary controls accurately gene expression finally recent advances technology that both highly multiplexed experiments signal amplication individual ta...