Helpful Hints for Better Aseptic Technique Aseptic technique is a set of principles and practices used by cell culture workers to reduce the potential of unwanted microorganisms or other cell lines from being introduced into: • Cell cultures • Sterile solutions and supplies • And, most importantly, the technician and coworkers. This is especially true when working with human cell lines known to contain oncogenic or infectious viruses or other harmful microorganisms (Barkley, 1979; Coecke et al., 2005). Good aseptic technique is essential for successful long-term cell and tissue cultures. Strict adherence to these principles and practices provides the following ...
Animal Cell Culture Guide Table of Contents This guide contains general technical information for working with animal cells in culture, including media, subculturing, cryopreserva- tion and contamination. A more comprehensive reference on animal cell culture can be found in Culture of Animal Cells: A Manual of Basic Technique, 5th edition, by R. Ian Freshney (24). Staying Safe in a Pandemic Environment ...............3 Culture Vessels and Surfaces ................................18 Materials, Contamination, and Workspace .................................... 3 Vessels ..............................................................................................18 Avoiding Infection ............................................................................. 3 Surface Coatings and Feeder Cells ...............................................20 Getting Started with an ATCC Cell Line ................4 Cryopreservation ................................................... 21 Product Sheet ................................................................................... 4 Overview ..........................................................................................21 Preparation of ...
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