Peanut Science zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA(1983) 10:93-97 zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA Specimen Preparation Techniques for Scanning Electron Microscopy of Developing Peanut Pegs' H. E. Pattee', S. C. Mohapatra3 and E. K. Agnello3 zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA ABSTRACT Key Words: Scanning electron microscopy, light microscopy, peanut fruit, maturation, development. This study evaluated various specimen preparation tech- niques for light microscopy (LM) and scanning electron micro- Anatomical studies of the peanut zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA(Arachis hypogaea zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA scopy (SEM). Main conclusions from this study were: (i) Critical point drying (CPD) was preferred over freeze dry- L.) fruit have generally been conducted on peanuts of a ing for SEM of whole or large pieces ...
protocol Preparation of cells for assessing ultrastructural localization of nanoparticles with transmission electron microscopy 1 1 2 1 Amanda M Schrand , John J Schlager , Liming Dai & Saber M Hussain 1 2 AFRL/711 HPW/RHPB, Wright-Patterson Air Force Base, Dayton, Ohio, USA. Department of Chemical Engineering, Case Western Reserve University, Cleveland, Ohio, USA. Correspondence should be addressed to S.M.H. (saber.hussain@wpafb.af.mil). Published online 25 March 2010; doi:10.1038/nprot.2010.2 otocolsWe describe the use of transmission electron microscopy (teM) for cellular ultrastructural examination of nanoparticle (np)- exposed biomaterials. preparation and imaging of electron-transparent thin cell sections ...
“Methods in Histology” Objectives: Understand the uses of the most important types of light microscopes Understand the basic operation and uses of electron microscopes Understand “resolution” and some basic units of size Understand basic steps in tissue preparation for light and electron microscopy Know major staining methods used in histology and what cell components are visualized with the different stains Understand basic principles and uses for other specific histological techniques, including enzyme histochemistry, immunohistochemistry, in situ hybridization, and autoradiography. Major types of Light Microscopy Brightfield : uses light focused on the specimen by a condenser lens, then brought ...
Nanoelectronics: the future of Electronics As transistor’s size becomes much smaller than micron, Microelectronics becomes Nanoelectronics (www.itrs.net/Common/2003ITRS/ExecSum2003.pdf ) 2 Challenges and Solutions • Challenge: preparation of a new generation of workers with solid skills in Nanoelectronics and Nanotechnology, overall • General approach to solution: acquiring the practical skills in Nanoelectronics through hands-on experience • Our specific solution: Interdisciplinary Nanoelectronics Laboratory for the Engineering/Science Undergraduate Curriculum 3 Scanning tunneling microscopy This novel technique yields surface topographies in real space and work function profiles on an atomic scale directly in real space. We know that the removal of ...
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