APPLI MICROBIOLOGY, May 1969, p. 707-709 Vol. 17, No. 5 Copyright @ 1969 American Society for Microbiology Printed in U.S.A. Tube Dilution Antimicrobial Susceptibility Testing: Efficacy of a Microtechnique Applicable to Diagnostic Laboratories LAWRENCE A. CHITWOOD Department ofPediatrics, Children's MemorialHospital, University ofOklahoma Medical Center, Oklahoma City, Oklahoma 73104 Received for publication 7 March 1969 Amicrotechnique for determining antibiotic susceptibilities by the serial dilution method was evaluated in a clinical diagnostic microbiology laboratory. As com- pared with the ...
Biomanufacturing: An inquiry lesson in growing cells Procedure for Counting Bacterial Cells via Serial Dilution Background: Bacterial cells are very small and can be difficult to count from a slide under a microscope. They also grow to a high density in a liquid culture, which can also make counting them rather tricky. When a bacterial culture in liquid media is introduced to an agar plate, each individual viable bacterium will form a colony, which ...
Serial Dilution Lab In this unit we will be discussing measurements of concentration that us e both very big and very small numbers. This exercise is intended to familiarize us with using scientific notation (to express big and small number easily) and the process of using serial dilutions to make concentrations of known values. Materials: FD&C Red Dye #40 (100% concentration) Dilution Well plate Dropper pipette Distilled water Crucible Ring stand and burner Procedure: 1. Put 10 drops ...
Serial Dilution Protocols | | Created: Friday, 30 September 2005 Author • Jackie Reynolds Information It is a common practice to determine microbial counts for both liquid and solid specimens---suspensions of E. coli in nutrient broth all the way to soil samples and hamburger meat. Most specimens have high enough numbers of microorganisms that the specimen has to be serially diluted to quantitate effectively.The following is a step-by-step procedure to working dilution problems, and includes some practice problems at ...
Practical Manual — EXPERIMENT 5 STANDARD PLATE Food Microbiology COUNT METHOD Structure 5.0 Objectives 5.1 Introduction 5.2 Principle 5.3 Materials Required 5.4 Procedure 5.4.1 E-coli Culture 5.4.2 Food Samples 5.5 Observations 5.6 Result 5.7 Precautions 5.0 OBJECTIVES After attending to this experiment, we shall be able to: • undertake enumeration of viable microorganisms by SPC; • perform the technique of serial dilution; • do pour plating; and ...
® SimPlate Total Plate Count Introduction ® SimPlate for Total Plate Count (TPC) method is used for the detection and quantification of the total aerobic ® microorganisms in food. The medium/sample mixture is dispensed into a SimPlate device and incubated for 24-28 h. The total aerobic plate count is determined by counting the fluorescent wells and referring to the ® ® SimPlate Conversion Table. The SimPlate device is packaged separately. Single Test Medium Multiple Test Medium Kit Components Kit Components ...
PDF | 0.67 MB | Free Download
