Rev. Salud Anim. Vol. 35 No. 1 (2013): 59-63 SHORT COMMUNICATION Evaluation of simplified DNA extraction methods for Streptococcus suis typing Ivette Espinosa, M. Baez, Maria Irian Percedo, Siomara Martinez ...
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...Rev salud anim vol no short communication evaluation of simplified dna extraction methods for streptococcus suis typing ivette espinosa m baez maria irian percedo siomara martinez division molecular biology national centre animal and plant health censa apdo san jose de las lajas mayabeque cuba e mail edu cu abstract is a gram positive bacterium that causes serious diseases in pigs humans with occupational risk the amplification gene fragments by pcr s may be complex expensive chemical reagents time consuming aim this study was to evaluate method rapid release genomic from colonies using physical o based on heating freezing case temperatures c were tested results showed directly at oc could useful an easy genotyping strains while not sufficient detection limit assay obtained purification ng considering size genome it possible estimate adequate amount cells are single colony ensure sensitivity key words direct evaluacion metodos simples extraccion adn para la tipificacion resumen es una ...