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...B sc hons ii year iv semester skill enhancement elective course experiments in cytology and genetics unit chromosome methodologies preparation of permanent slide mounting tissue section squash smear a suitable medium e g canada balsam euparal after staining it is necessary to avoid drying up not render opaque the media should have refractive index closer glass refraction harden quickly contact with air check de principal aims are i transparent increase visibility under microscope iii hold protecting coverslip firmly place preserve for long period sections smears crystal violet dehydration passed through absolute ethanol keeping each sec differentiation done by passing clove oil min observation required satisfactory then transferred kept clearing xylol hour finally mounted allowed dry overnight on hot plate c squashes aceto orcein carmine feulgen acetic alcohol schedule paraffin seal temporary preparations carefully removed blade days inverted covered petridish containing glacial acid m...