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...Validation of human cell counting methods daniella paiva bonfim bruno cosme jailton carreteiro damasceno jose mauro granjeiro and fernanda leve division metrology applied to life sciences dimav national institute quality technology inmetro brazil materials dimat corresponding author fleve gov br abstract the quantification biological processes has gained importance in as its application diagnosis personalized therapy been increasing demand biotechnology sector considering that is microscopic functional unit living beings count critical for reproducibility vitro study this work aims determine measurement uncertainty commutability manual a neubauer chamber automatic both image based widely used we different amounts x hct cells well established line whose authenticity was confirmed by str analysis initially checked if up down movements performed with micropipette are enough maintain homogeneous distribution four most outer quadrants then were prepared using balance or calibrated micropipe...