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...Author peter d pioli date edited protocol hemocytometer cell counting application manual of suspensions procedure aliquot l trypan blue solution into appropriately labeled tube pre mixed suspension the from step a ratio to equals fold dilution vortex briefly touch spin if using ml microfuge tubes bring contents down lid pipette chamber between notch and glass coverslip figure black arrow count cells in boxes b red calculate total your sample formulas below factor x volume example counted or overview view with denotes used for loading samples illustration area general are may change based upon predicted concentration aim box then less can be assuming equal distribution across avoid overestimation numbers include that lie on top right edges each do not along bottom left...