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...Asmita et al sgvu journal of pharmaceutical research education review article homepage http www gyanvihar org researchjournals plant tissue culture a v gaikwad dr s k singh ritu gilhotra school pharmacy abstract refers to growing and multiplication cells tissues organs plants on defined solid or liquid media under aseptic controlled environment the commercial technology is primarily based micro propagation in which rapid proliferation achieved from system cuttings axillary buds limited extent somatic embryos cell clumps suspension cultures bioreactors introduction whole can be regenerated small suitable medium plantlets so produced are called raised technique organism artificial prepared nutrient static conditions characterized by disease free growth more fibrous healthier root bushier branching habit higher survival rate history was first proposed german botanist golliob haberlandt he regarded as father mainly worked palisade grew them knob salt solution with sucrose observed hanning ...