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...Int j adv res biol sci international journal of advanced research in biological sciences issn www ijarbs com a peer reviewed referred indexed and open access doi coden ijarqg usa volume issue reviewarticle http dx org application recombinant dna technology agriculture review rupaverma pushpa kumari sneh megha ladly rani m sc biotechnology under pg department botany ranchi university jharkhand india corresponding author drrupav gmail rupavermabiotech abstract food is very imported requirement our life its demand shall keep on increasing with the increase population all over world but productivity reduced because many challenges production only plant breeding methods cannot address serious during mid s traditional crop improvement like selection process plants for yield resistance to different diseases draught were practice nowadays there are new techniques used gene transfer cell protoplast culture etc due this technique transgenic resistant disease predators drought even can be grown w...