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...Contents i introduction compare optical microscope and electron localization method storm stochastic reconstruction microscopy ii experiments results discussion three dimensional evaluation of spatial resolution d imaging microtubules in a cell clathrin coated pits iii summary time measurement condition s surfaceinterface near surface nm ms internal solid or liquid super not need drying several tens living single molecule tracking x pixel y f iexp bg sx sy cos sin actual precision high technique each diffraction limit the overall image is then reconstructed from fluorophore positions obtained multiple cycles for photo switchable needed reporter that can be cycled between fluorescent dark states alexa off state on activator facilitates activation cy combinatorial pairing reporters activators allows creation probes with many distinct colors...