Common PCR-based Genotyping Methods for SNP Analysis SNPs can have up to 4 alleles (A/C/G/T), but two alleles are most common. These methods can only positively detect one allele. • PCR-RFLP / CAPS - Polymerase Chain Reaction - Restriction Fragment Length Polymorphisms - Also called Cleaved Amplified Polymorphic Sequences (CAPS) - A Single Nucleotide Polymorphism (SNP) where one allele creates (or removes) a naturally occurring restriction site. Amplifying the sequence surrounding these SNPs from individuals, cutting the products with a restriction enzyme and resolving on a gel will reveal which alleles an individual carries. • dCAPS - derived Cleaved Amplified ...
It is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives .It is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA samples in question with specific restriction endonucleases. RFLP, as a molecular marker, is specific to a single clone/restriction enzyme combination. Each organism inherits its DNA from its parents. Since DNA is replicated with each generation, any given sequence can ...
DNA EXTRACTION METHODS DNA extraction is used to isolate Types of DNA Mitochondrial DNA Genomic DNA Plasmid DNA How Can We Recover DNA From a Variety of Sources of Biological Evidence? Blood, Tissues, Saliva, Urine, Hair (w/Root & Shaft), Teeth, Bone, Semen, Cigarette Butts, Envelope & Stamps, Fingernail Clippings, Chewing Gum, Feces Purpose of DNA Extraction To obtain DNA in a relatively purified form which can be used for further investigations such as: PCR (polymerase chain reaction) RFLP (restriction fragment length polymorphism) Southern Blotting What are the essential components of a DNA extraction Procedure? 1. Maximize DNA recovery ...
Content Forensic Science and DNA fingerprinting Invention of DNA fingerprinting Preparation of DNA Fingerprint Steps of DNA fingerprinting Types of Analysis RFLP, PCR, STR Analysis, AFLP Analysis Applications DNA Fingerprinting and Forensic Science Forensic science Intersection of Law and Science Historic examples of collecting evidences: 18th Century – Photography Obsolete & Can Early 19th Century – Fingerprints Be Manipulated From 1985 – DNA Fingerprinting Unique signature found in each person’s genetic makeup Invention of DNA Fingerprinting Invented by Alec Jeffreys at the University of Leicester in 1985. DNA Fingerprinting Different from Full Genome Sequencing DNA Fingerprinting ...
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