Sterilization & Disinfection Sterilization & Disinfection Sterilization: Is the killing or removal of all micro-organisims, including bacterial spores, which are highly resistant. e.g.: - o Sterilization by autoclave (121 c , 15psi). - Ethyl oxide gas for surgical instruments. - Filtration for intravenous solution. Classification of sterilization agents: 1- Physical agents: a- heat b- filtration c- radiation 2- Chemical agents: Classification of Sterilization Methods Classification of Sterilization Methods Physical Sterilization Chemical Sterilization Radiation Filtration Heat Direct Sun ...
Objectives : 1- Define the terms sterilization, disinfectant and antiseptic. 3 2- Classify the different methods of sterilization (physical and chemical methods). 4 3- Know and realizes that heat is the most important method of sterilization and its application in medical practice. 5 4- Know dry heat as applied in hot air Oven and moist heat as applied in autoclaves. 7 5- Know the principles of Autoclave function and monitoring methods of sterilization .6-9 6- Know the importance of non ...
Sterilization: Is the killing or removal of all micro-organisims, including bacterial spores, which are highly resistant. e.g.: - o Sterilization by autoclave (121 c , 15Klb). - Ethyl oxide gas for surgical instruments. - Filtration for intravenous solution. Classification of sterilization agents: 1- Physical agents: a- heat b- filtration c- radiation 2- Chemical agents: 1- Physical agents: A- Heat: Most effective cause it effectively stops cellular activates by coagulate proteins or oxidize cell components. Types of sterilization by heat: ...
Sterilization concept • Sterilization is essential concept in the preparation of sterile pharmaceutical products . • Its aim is to provide a product that is safe and eliminates the possibility of introducing Sterilization Sterilization –a process that by which all viable M.O are removed or destroyed , based on a probability .function Method of inactivating M.O :may be classified as either 1.Physical A. moist heat b. dry heat c. irradiation d. sterile filtration is another process , but it ...
Hot air oven (Dry heat Sterilizer) Materials sterilized: o Glassware - syringes, petri dishes, flasks, pipettes and test tubes. o Surgical instruments - scalpels, forceps, etc. o Chemicals such as liquid paraffin, fats, glycerol, oil, and glove powder, etc. Hot air oven - precautions Overloading of hot air oven should be avoided. Material should be arranged in a manner so that free circulation of air is maintained. Material to be sterilized should be dried completely. Cotton plugs should be used ...
INTRODUCTION Sterilization A physical or chemical process that completely destroys or removes all microbial life, including spores. Disinfection It is killing or removing of harmful microorganisms Disinfectant Products used to kill microorganisms on inanimate objects or surfaces. Disinfectants are not necessarily sporicidal, but may be sporostatic, inhibiting germination or outgrowth Antiseptic A product that destroys or inhibits the growth of microorganisms in or on living tissue. Aseptic Characterized by the absence of pathogenic microbes. METHODS OF STERILIZATION 1. Physical methods ...
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