DNA EXTRACTION METHODS DNA extraction is used to isolate Types of DNA Mitochondrial DNA Genomic DNA Plasmid DNA How Can We Recover DNA From a Variety of Sources of Biological Evidence? Blood, Tissues, Saliva, Urine, Hair (w/Root & Shaft), Teeth, Bone, Semen, Cigarette Butts, Envelope & Stamps, Fingernail Clippings, Chewing Gum, Feces Purpose of DNA Extraction To obtain DNA in a relatively purified form which can be used for further investigations such as: PCR (polymerase chain reaction) RFLP ...
Overview Objectives Background information Materials Procedures: Part 1: Restriction of Lambda DNA Part 2: Gel Electrophoresis of Lambda DNA Part 3 : DNA Extraction Part 4: Chemical Tests Closing and Feedback Objective Restrict Lambda DNA and analyze by Gel Electrophoresis Extract a sizable amount of DNA Understand and use basic biochemical techniques: DNA isolation method Gel electrophoresis Biochemical tests Understand role of ingredients used in experiment Plant Cell Structure Plant Cell Structure: The Nucleus Nuclear envelope Double layer of lipids ...
Instructors - Bio-Rad Curriculum and Training Specialists Sherri Andrews, Ph.D., Eastern US sherri_andrews@bio-rad.com Damon Tighe, Western US damon_tighe@bio-rad.com Leigh Brown, M.A., Central US leigh_brown@bio-rad.com 2 Biotechnology Explorer™ | explorer.bio-rad.com Workshop Timeline Introduction Background on DNA Extraction Extract Genomic DNA from Cheek Cells Prepare DNA Necklaces 3 Biotechnology Explorer™ | explorer.bio-rad.com Relevance of DNA Isolation Isolation of DNA is often the first step before further analysis – DNA profiling – Cloning &ndash ...
• Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. • The DNA extraction process requires careful handling of biological material to prevent sample contamination and crossover. Tubes should be carefully labelled, especially when transfers are required. • The simplest cells, such as bacteria cells, are prokaryotes. These prokaryotes comprise a lipid bilayer outer membrane and a cytoplasm containing a ...
DNA Extraction Gel Electrophoresis • Because nucleic acids are negatively charged ions at neutral or basic pH in an aqueous environment, they can be mobilized by an electric field. Gel electrophoresis is a technique used to separate molecules on the basis of size, using this charge. PCR • PCR is used to amplify a specific sequence of DNA. Reverse Transcriptase PCR DNA fragments can also be amplified from an RNA template in a process called reverse transcriptase PCR ...
PPT - Power Point | 0.32 MB | Free Download
