Sterilization & Disinfection Sterilization & Disinfection Sterilization: Is the killing or removal of all micro-organisims, including bacterial spores, which are highly resistant. e.g.: - o Sterilization by autoclave (121 c , 15psi). - Ethyl oxide gas for surgical instruments. - Filtration for intravenous solution. Classification of sterilization agents: 1- Physical agents: a- heat b- filtration c- radiation 2- Chemical agents: Classification of Sterilization Methods Classification of Sterilization Methods Physical Sterilization Chemical Sterilization Radiation Filtration Heat Direct Sun Light Non Ionizing Radiation Moist Heat Dry Heat Ionizing Radiation Infrared Radiation Flaming Sterilization at a temp. Below 100Co o ...
Objectives : 1- Define the terms sterilization, disinfectant and antiseptic. 3 2- Classify the different methods of sterilization (physical and chemical methods). 4 3- Know and realizes that heat is the most important method of sterilization and its application in medical practice. 5 4- Know dry heat as applied in hot air Oven and moist heat as applied in autoclaves. 7 5- Know the principles of Autoclave function and monitoring methods of sterilization .6-9 6- Know the importance of non heat sterilization methods and their use for sterilization of heat sensitive objects. 13-15 7-Know the difference between antiseptics and disinfectants ...
Sterilization concept • Sterilization is essential concept in the preparation of sterile pharmaceutical products . • Its aim is to provide a product that is safe and eliminates the possibility of introducing Sterilization Sterilization –a process that by which all viable M.O are removed or destroyed , based on a probability .function Method of inactivating M.O :may be classified as either 1.Physical A. moist heat b. dry heat c. irradiation d. sterile filtration is another process , but it only removes , not inactivates M.O Method of inactivating M.O may : be classified as either 2. Chemical a ...
INTRODUCTION Sterilization A physical or chemical process that completely destroys or removes all microbial life, including spores. Disinfection It is killing or removing of harmful microorganisms Disinfectant Products used to kill microorganisms on inanimate objects or surfaces. Disinfectants are not necessarily sporicidal, but may be sporostatic, inhibiting germination or outgrowth Antiseptic A product that destroys or inhibits the growth of microorganisms in or on living tissue. Aseptic Characterized by the absence of pathogenic microbes. METHODS OF STERILIZATION 1. Physical methods Heat Dry Moist Radiation U.V. light Ionizing radiation Filtration 2. Chemical Methods MODES OF ACTION OF STERILANTS AND DISINFECTANTS ...
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