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picture1_Spectrophotometry Slideshare 66830 | Spectrophotometry Calculations Aalbati


 158x       Filetype PPTX       File size 0.23 MB       Source: fac.ksu.edu.sa


File: Spectrophotometry Slideshare 66830 | Spectrophotometry Calculations Aalbati
spectrophotometry spectrophotometry is the measurement of light absorption or transmission it is an analytical technique that is applied to obtain valuable information such as the identity of an unknown compound ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago
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...Spectrophotometry is the measurement of light absorption or transmission it an analytical technique that applied to obtain valuable information such as identity unknown compound by their characteristic spectra qualitative analysis and determination concentration analyte quantitative used for both enzyme catalyzed reactions can be followed measuring substrate product regions electromagnetic spectrum spectrophotometer a instrument measure amount transmitted absorbed sample components include source collimator focusing device transmits intense beam monochromator divides into its component wave lengths selector selecting desired wavelength compartment in which placed cuvette photodetector electrical meter record output detector beer lambert law fraction incident solution depends on thickness path length l absorbing c chemical nature relationship between substance expressed mathematically log i absorbance optical density o d coefficient extinction particular follows exponential rather than ...

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