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...Pertanika j sci technol science technology journal homepage http www upm edu my evaluation of factors affecting microbial growth inhibition and optimization using pineapple leaves juice norazwina zainol amirah ya acob putri nurul yasmin mohd ridza siti hatijah mortan kamaliah abdul samad college engineering universiti malaysia pahang lebuhraya tun razak ump gambang kuantan faculty chemical process abstract this study optimized conditions leaf plj the sugarcane press machine was used to considered four be analyzed by two level factorial design tlfd which are time h concentration total phenolic content tpc mg gae ml temperature c ratio microbe m v colony forming unit cfu method employed measure expressed as a percent in terms central composite ccd experimental created response surface methodology rsm determined optimum min best were analysis variance anova showed that factor has greatest contribution inhibiting article info accompanied history b with received june accepted january publis...