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...May grunwald mg staining of blood smears this protocol describes standard pattern erythrocytes pale pink thrombocytes small and blue lymphocytes round nucleus almost filling the whole cell neutrophiles segmented within cytoplasm eosinophils granules in basophils monocytes preparation are made from well mixed fresh total edta or resuspended buffy coat if enrichment leukocytes is required pipet l middle specimen slide untreated for coated ihc ca cm labeling area bring a clean spreader held at angle toward drop on wait until spreads along entire width while holding same push it forward rapidly smoothly without applying any force increasing makes shorter decreasing longer smear dry least min rt before dried be stored several days use optional aequous procedures will used fix cold meoh air proceeding since solution up prior fixation not necessary place flat surface stain leave dilute by adding mm napi lift to drain tray with ho vertically mount coverslips using an based mounting medium mate...