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...Southeast asian fisheries development center aquaculture department seafdec aqd institutional repository http org ph government of japan trust fund goj tf laboratory manuals disk diffusion method tendencia eleonor a e in manual standardized methods for antimicrobial sensitivity tests bacteria isolated from aquatic animals and environment pp tigbauan iloilo philippines hdl handle net downloaded s chapter diskdiffusion principle this is based on the that antibiotic impregnated placed agar previously inoculated with test bacterium pick up moisture diffuse radially outward through medium producing an concentration gradient at edge high gradually diminishes as distance increases to point where it no longer inhibitory organism which then grows freely clear zone or ring formed around after incubation if agent inhibits bacterial growth media performed using mueller hinton mha best routine susceptibility because has good reproducibility low sulfonamide trimethoprim tetracycline inhibitors gives...