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...Diagn microbiol infect dis mycology use of lactophenol cotton blue mounts corneal scrapings as an aid to the diagnosis mycotic keratitis philip a thomas kuriakose mothi p kirupashanker and v senthil maharajan lpcb ulcer scrap culture positive patients negative results in ings were assessed diagnostic tool cases ker there was sig atitis over period months we investigated nificant difference between percentage ulcerative by microbiological techniques consisting obtained gram stained smears direct microscopic examination mount well material scraped from due fusarium spp asper fungi isolated large numbers on multiple media gillus is strongly recommended proven my simple rapid inexpensive sensitive technique cotic yielded important problem faced but these methods suffer certain drawbacks ophthalmologists especially developing coun forster et al other staining such tries johns o day de periodic acid schiff pas or grocott s me lay institution specific antifungal therapy thenamine silver gms e...