fluorescence microscopy image of african green monkey kidney fibroblast cells simultaneous labelling of different parts of a cell with different fluorophores red filamentous actin network green lectin binding to the ...
Filetype Power Point PPTX | Posted on 09 Sep 2022 | 4 years ago
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...Fluorescence microscopy image of african green monkey kidney fibroblast cells simultaneous labelling different parts a cell with fluorophores red filamentous actin network lectin binding to the golgi apparatus blue dna in nucleus fluorescent protein occurs naturally jellyfish structure gfp is beta barrel chromophore centre variant proteins have been developed slightly structures and peak wavelengths fusion can be expressed many organisms used visualize or kinds leaf illuminates muscle chloroplasts controlling nerve c elegans forster resonance energy transfer fret donor molecule excited emit transmitted acceptor if they are close emits at longer wavelength measure both emissions high signal means far apart possible pairs would cy two types there needs overlap between emission spectrum excitation three uses example from blanchard paper on trnas ribosome...