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...Contents page restriction enzymes gel electrophoresis blotting techniques southern northern and western dna sequencing polymerase chain reaction pcr recombinant gene cloning references recommended reading nuclease an enzyme that cleaves a molecule of at any site where specific short sequence nucleotides occurs key types endonuclease within the exonuclease ends endonucleases classified on subunit composition cleavage position specify co factor requirement type ii is main one used in two terms recognition sites nucleotide recognised phosphodiester bond cleaved make break each stands double helix gaattc cttaag g aattc cttaa...